CJC-1295 DAC
From $31.50to $183.75
CJC-1295 with DAC (drug affinity complex), lyophilized reference standard for in vitro laboratory research.
Price (/ pack)
$183.75
Total
10 total vials
$183.75
✓ Total Savings: You save $166.25 (25% total savings vs regular price)
Quantity
In Stock · 10 Units Available
This strength’s Certificate of Analysis isn’t published yet — add to cart will be enabled once the lab report is available.
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Certificate of Analysis & Specifications
Documentation will be published once this lot completes third-party analytical release.
Released COA Library
Published Certificates of Analysis for CJC-1295 DAC. All released analytical records are preserved here. Pending and in-testing lots are reachable only through the QR printed on the vial.
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Reports appear here once a batch has completed third-party analytical release.
Related research materials
Research Data
Chemical & Structural Identifiers
- Chemical Name (IUPAC)
- Nε 30-[3-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)propanoyl]-[D-Ala2, Gln8, Ala15, Leu27]-GRF(1-29)-Lys30-amide
- CAS Registry Number
- 446262-90-4 (the unconjugated 29-residue GRF(1-29) backbone is cataloged separately as 863288-34-0)
- PubChem CID
- 91971820
- Molecular Formula
- C165H269N47O46
- Molecular Weight
- 3644.02 Da (3647.25 g/mol average)
- Sequence
- Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-Lys(MPA)-NH2
- SMILES
- CC[C@H](C)[C@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@@H](NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H](C)NC(=O)[C@H](Cc3ccc(O)cc3)N)[C@@H](C)O)C(=O)NC(CC(C)C)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCCNC(=O)CCN4C(=O)C=CC4=O)C(=O)N
- InChIKey
- UNQVYJUSWLZTQD-NEXMOMPOSA-N
- Salt / Counter-Ion
- Supplied as a lyophilized acetate (CH3COO-) or trifluoroacetate (CF3COO-) salt.
- Synonyms
- CJC-1295 with DAC, DAC:GRF, CJC-1295 DAC, Maleimidopropionyl-Mod GRF(1-29)
- Registry Cross-Reference: PubChem CID 91971820 corresponds to the 30-residue DAC-conjugated sequence and to the formula C165H269N47O46 given above. Registry records for this compound family apply the CJC-1295 name across both the conjugated and backbone-only forms, so verify the batch against its Certificate of Analysis rather than the trade name alone.
- Variant Distinction: This entry describes the DAC-conjugated 30-residue form, which carries a 3-maleimidopropionyl group on Lys30. The unconjugated 29-residue analog has a lower molecular mass and is cataloged separately; the two are not interchangeable in an assay.
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.5% area normalization under UV detection at λ = 214 nm and 220 nm.
- Identity Confirmation (RP-HPLC RT + UV vs. reference)
- Main peak retention time and UV spectrum conform to the reference standard.
- Physical Characterization
- High-density, white to off-white lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light.
Solubility. Soluble in sterile laboratory-grade deionized water (≥ 10 mg/mL), sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 5 mg/mL), or dilute 0.1% acetic acid for stock stabilization in in vitro assay applications.
- Maleimide Preservative Handling: The C-terminal maleimide group undergoes rapid nucleophilic addition with free thiols; *do not* reconstitute in buffers containing exogenous reducing agents (e.g., DTT, β-mercaptoethanol, or L-cysteine) unless intentionally conducting bioconjugation reaction assays. Aliquot into single-use fractions to eliminate freeze-thaw degradation cycles.
Mechanism & Literature
Potent Growth Hormone-Releasing Hormone Receptor (GHRHR) agonist engineered with a 3-maleimidopropionic acid (Drug Affinity Complex / DAC) linker at Lys30. In in vitro or biochemical assay media, the maleimide moiety undergoes a spontaneous, selective covalent reaction (Michael addition) with the unpaired cysteine thiol (Cys34) of serum albumin (BSA/HSA).
Cellular Pathways Investigated: Evaluated in primary somatotroph cultures and GHRHR-expressing cell lines to quantify intracellular cyclic AMP (cAMP) accumulation, adenylyl cyclase activation, and stimulation of growth hormone gene transcription (GH1) while bound to albumin adducts. Assays demonstrate selective activation of the pituitary growth hormone axis without inducing non-specific transcription of cortisol or prolactin.
References (3)
- Teichman, S. L., et al. (2006). Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults. The Journal of Clinical Endocrinology & Metabolism, 91(3), 799–805.
- Jette, L., et al. (2005). hGRF1-29-albumin bioconjugates activate the GHRH receptor on anterior pituitary cells in vitro and resist rapid enzymatic degradation. Endocrinology, 146(7), 3052–3058.
- Database record: National Center for Biotechnology Information. PubChem Compound Summary for CID 91971820, CJC-1295. PubChem, U.S. National Library of Medicine. Registry cross-reference for CAS 446262-90-4 (record reviewed 2026-09-15).
Technical Laboratory FAQs
How does CJC-1295 with DAC differ chemically from CJC-1295 No DAC (Mod GRF 1-29)?
While both share the identical tetrasubstituted 29-amino acid GHRH backbone (D-Ala2, Gln8, Ala15, Leu27), CJC-1295 DAC contains an additional C-terminal lysine residue functionalized with a 3-maleimidopropionyl group (DAC) for covalent bioconjugation to albumin.
What is the function of the Drug Affinity Complex (DAC) in laboratory assays?
The maleimide functionality on DAC specifically forms a covalent bond with the free sulfhydryl group of Cys34 on albumin, protecting the peptide from enzymatic cleavage by dipeptidyl peptidase-4 (DPP-4) and renal filtration in pharmacokinetic models.
What reconstitution vehicle is recommended for in vitro assay modeling of CJC-1295 DAC?
Sterile deionized water or sterile PBS (pH 7.4) is recommended. Assay buffers must remain free of extraneous thiol-containing reducing reagents to prevent premature quenching of the maleimide reactive group before intended albumin-binding experiments.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.