Quantity
Price
$27.00
Out of stock
DSIP
From $27.00to $277.50
For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.
This item is available only through approved account access.
Product Specifications
- Lot Number
- —
- Purity
- —
- Storage Temp
- 36°F to 46°F
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.
Documentation
Certificates of Analysis are supplied with each product lot where applicable.
Certificate of Analysis (COA)
Documentation will be available once product lots are received and tested.
All products currently listed on this site are for research purposes only.
COA Library
Published Certificates of Analysis for DSIP. New deliveries are added as their batches are released.
No released COA for this strength yet
Reports appear here once a batch for this strength has been released.
Research Data
Chemical & Structural Identifiers
- Chemical Name (IUPAC)
- L-tryptophyl-L-alanyl-L-α-aspartylglycyl-L-α-glutamyl-L-alanyl-L-serylglycyl-L-glutamic acid
- CAS Registry Number
- 62568-57-4
- PubChem CID
- 43817
- Molecular Formula
- C35H48N10O15
- Molecular Weight
- 848.33 Da (848.81 g/mol average)
- Sequence
- H-Trp-Ala-Asp-Gly-Glu-Ala-Ser-Gly-Glu-OH (Single-letter: WADGEASGE)
- SMILES
- CC(C(=O)NC(CC(=O)O)C(=O)NCC(=O)NC(CCC(=O)O)C(=O)NC(C)C(=O)NC(CO)C(=O)NCC(=O)NC(CCC(=O)O)C(=O)O)NC(=O)C(CC1=CNC2=CC=CC=C21)N
- InChIKey
- DVBWNWVLUSVDEQ-QNNHHSAWSA-N
- Salt / Counter-Ion
- Supplied as a high-purity lyophilized acetate (CH3COO-) or trifluoroacetate (CF3COO-) salt.
- Synonyms
- DSIP, Delta Sleep-Inducing Peptide, Emideltide, Nonapeptide DSIP
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.5% by area normalization under UV detection at λ = 214 nm and 280 nm (indole detection).
- Identity Confirmation (ESI-MS / MALDI-TOF)
- Theoretical mass [M+H]+ = 849.3 Da; observed mass-to-charge ratio m/z = 849.3 ± 0.5 Da.
- Residual Moisture (Karl Fischer Titration)
- ≤ 2.0% water content.
- Endotoxin Screening (LAL Assay)
- < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
- Sterility Validation
- USP <71> compliant (no observed microbial proliferation).
- Physical Characterization
- Dense, white to off-white lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, sealed under an inert gas matrix and protected from direct ambient light.
Solubility. Highly soluble in sterile laboratory-grade deionized water (≥ 15 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 10 mg/mL) for in vitro assay application.
- Photostability & Handling: The N-terminal tryptophan residue is prone to photo-oxidation; prepare stock solutions under amber light conditions, avoid excessive high-shear vortexing, and aliquot into single-use fractions at -20°C to prevent degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Structural Origin & Biochemical Discovery: Endogenous nonapeptide initially isolated from the cerebral venous blood of lagomorph models undergoing low-frequency electrical thalamic stimulation, demonstrating selective electrophysiological neuromodulatory characteristics in preclinical literature.
Cellular Pathways Investigated: Evaluated in in vitro hypothalamic explants, pituitary cell cultures, and neuronal assays to monitor modulation of monoaminergic neurotransmission (serotoninergic and GABAergic metabolic turnover), suppression of corticotropin-releasing factor (CRF)-stimulated adrenocorticotropic hormone (ACTH) secretion, and upregulation of antioxidant enzyme transcription (superoxide dismutase [SOD] and catalase activity) under induced oxidative stress modeling.
References (3)
- Schoenenberger, G. A., & Monnier, M. (1977). Characterization of a delta-electroencephalogram(-sleep)-inducing peptide. Proceedings of the National Academy of Sciences (PNAS), 74(3), 1282–1286.
- Graf, M. V., & Kastin, A. J. (1984). Delta-sleep-inducing peptide (DSIP): an overview of central and peripheral actions in vitro and in vivo. Peptides, 5(6), 1165–1174.
- Iyer, K. S., et al. (1988). In vitro effects of delta sleep-inducing peptide (DSIP) on corticotropin secretion from rat anterior pituitary cells. Life Sciences, 43(16), 1321–1326.
Technical Laboratory FAQs
What is the primary sequence and molecular mass of DSIP?
DSIP is a synthetic linear nonapeptide with the primary sequence Trp-Ala-Asp-Gly-Glu-Ala-Ser-Gly-Glu (WADGEASGE) and a molecular mass of 848.81 g/mol.
Why does in vitro preparation of DSIP require light-protective handling?
The N-terminal Trp1 residue contains an electron-rich indole side chain susceptible to light-induced oxidation in aqueous buffers, generating analytical artifacts and altered chromatographic retention times.
What solvent matrix is recommended for reconstituting DSIP in laboratory assays?
Sterile deionized water or sterile PBS (pH 7.4) provides immediate dissolution at concentrations exceeding 10 mg/mL, creating a neutral delivery carrier for cell culture and biochemical modeling.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.