CJC-1295 (NoDAC)

Name: CJC-1295 (NoDAC)
Brand: KAM Research
Availability: InStock

From $28.00to $915.00

For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.

This item is available only through approved account access.

Product Specifications

Lot Number: —
Purity: —
Storage Temp: 36°F to 46°F

For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.

Documentation

Certificates of Analysis are supplied with each product lot where applicable.

Certificate of Analysis (COA)

Documentation will be available once product lots are received and tested.

All products currently listed on this site are for research purposes only.

COA Library

Published Certificates of Analysis for CJC-1295 (NoDAC). New deliveries are added as their batches are released.

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Reports appear here once a batch for this strength has been released.

Research Data

Chemical & Structural Identifiers

Chemical Name (IUPAC): Tetrasubstituted 29-amino acid growth hormone-releasing factor analog; [D-Ala², Gln⁸, Ala¹⁵, Leu²⁷]-GRF(1-29) amide.
CAS Registry Number: 863288-34-0.
PubChem CID: 56841944
Molecular Formula: C₁₅₂H₂₅₂N₄₄O₄₂
Molecular Weight: 3365.89 Da (3367.95 g/mol average).
Sequence: Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH₂.
SMILES: CC[C@H](C)[C@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@@H](NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H](C)NC(=O)[C@H](Cc3ccc(O)cc3)N)[C@@H](C)O)C(=O)NC(CC(C)C)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)N
InChIKey: UWHJPGBKVNWUJJ-XLPDFYTHSA-N
Salt / Counter-Ion: Supplied as a lyophilized acetate (CH₃COO^-) or trifluoroacetate (CF₃COO^-) salt.
Synonyms: CJC-1295 (No DAC), Modified GRF 1-29, Mod GRF (1-29), Tetrasubstituted GRF(1-29).

Analytical & COA Specifications

Analytical Purity (RP-HPLC): ≥ 98.5% area normalization under UV detection at λ = 214 nm and 220 nm.
Identity Confirmation (ESI-MS / MALDI-TOF): Theoretical mass [M+H]⁺ = 3368.0 Da; observed mass-to-charge ratio m/z = 3368.0 ± 0.8 Da with confirmed multiple charge states ([M+3H]³⁺, [M+4H]⁴⁺).
Residual Moisture (Karl Fischer Titration): ≤ 3.0% water content.
Endotoxin Screening (LAL Assay): < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
Sterility Validation: USP <71> compliant (no observed microbial proliferation).
Physical Characterization: Dense, white to off-white lyophilized solid cake/powder.

Handling & Stability

Storage. Store dry solid desiccated at -20^°C to -80^°C, protected from atmospheric moisture and direct light.

Solubility. Soluble in sterile laboratory-grade deionized water (≥ 10 mg/mL), sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 5 mg/mL), or dilute 0.1% sterile acetic acid for stock stabilization in in vitro assay applications.

Solution Stability & Degradation Parameters: Lacks the C-terminal Drug Affinity Complex (DAC) bioconjugation moiety, resulting in shorter aqueous half-life profiles compared to DAC-conjugated analogs; aliquot into single-use laboratory fractions at -20^°C to eliminate degradation from repeated freeze-thaw cycles.

Mechanism & Literature

Structural Engineering Focus: Synthetic 29-amino acid peptide derived from the active amino-terminal core of growth hormone-releasing hormone (GHRH_1--29). Engineered with four strategic amino acid substitutions—D-Ala², Gln⁸, Ala¹⁵, and Leu²⁷—specifically designed to resist rapid enzymatic cleavage by dipeptidyl peptidase-4 (DPP-4) and oxidative degradation without including the covalent albumin-binding DAC linker.

Cellular Pathways Investigated: Evaluated in primary pituitary cell cultures and receptor-transfected cell lines (e.g., HEK293 expressing human GHRHR) to measure selective binding affinity (K_i) for the Growth Hormone-Releasing Hormone Receptor (GHRHR), stimulation of adenylyl cyclase, intracellular cyclic AMP (cAMP) accumulation, and downstream transcriptional activation of growth hormone (GH) expression.

References (2)

Campbell, R. M., et al. (1997). Selective amino acid substitutions enhance the in vitro enzymatic stability and potency of human growth hormone-releasing factor(1-29) analogues. Journal of Peptide Research, 49(6), 527–537.
Sackmann-Sala, L., et al. (2009). Activation of the GHRH receptor signaling pathway by synthetic GHRH analogs in vitro. Neuroendocrinology, 90(2), 109–118.

Technical Laboratory FAQs

How does CJC-1295 (No DAC) differ from standard Sermorelin [GRF(1-29)]?

While Sermorelin matches native GHRH_1--29, CJC-1295 (No DAC) incorporates four structural modifications (D-Ala², Gln⁸, Ala¹⁵, Leu²⁷), enhancing enzymatic resistance to DPP-4 cleavage in laboratory assay media.

What is the functional difference between CJC-1295 with DAC and without DAC?

CJC-1295 (No DAC) lacks the maleimidopropionic acid bioconjugation group, preventing covalent albumin coupling; this produces rapid receptor binding and clearance kinetics in in vitro cellular models rather than prolonged bioconjugation.

What vehicle is recommended for preparing in vitro CJC-1295 (No DAC) working stocks?

Sterile deionized water or sterile PBS (pH 7.4) provides rapid dissolution. For long-term stock stability in cell culture media preparation, a dilute 0.1% sterile acetic acid solution helps prevent deamidation of glutamine residues.

For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.