Quantity
Price
$33.00
Out of stock
TB-500
From $33.00to $787.50
For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.
This item is available only through approved account access.
Product Specifications
- Lot Number
- —
- Purity
- —
- Storage Temp
- 36°F to 46°F
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.
Documentation
Certificates of Analysis are supplied with each product lot where applicable.
Certificate of Analysis (COA)
Documentation will be available once product lots are received and tested.
All products currently listed on this site are for research purposes only.
COA Library
Published Certificates of Analysis for TB-500. New deliveries are added as their batches are released.
No released COA for this strength yet
Reports appear here once a batch for this strength has been released.
Research Data
Chemical & Structural Identifiers
- Chemical Name (IUPAC)
- N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-threonyl-L-glutamyl-L-threonyl-L-glutamine (thymosin β4 active fragment)
- CAS Registry Number
- 77591-33-4
- Molecular Weight
- ≈ 888.5 Da (LKKTETQ fragment, monoisotopic; observed [M+H]⁺ = 889.5 Da)
- Sequence
- Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln (single-letter: Ac-LKKTETQ, thymosin β4 residues 17-23)
- Salt / Counter-Ion
- Supplied as a high-purity lyophilized acetate (CH₃COO⁻) or trifluoroacetate (CF₃COO⁻) salt.
- Synonyms
- TB-500, Thymosin beta-4 fragment, Thymosin β4 (17-23), LKKTETQ, Thymosin β4 active fragment
- The designation TB-500 refers to the synthetic N-terminal active fragment (Ac-LKKTETQ) of thymosin β4. Some vendor listings apply the same CAS (77591-33-4) to full-length thymosin β4 (4963.5 Da); confirm the exact fragment length against the batch Certificate of Analysis before use.
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.0% area normalization under UV detection at λ = 214 nm and 220 nm.
- Identity Confirmation (ESI-MS / MALDI-TOF)
- Observed fragment mass [M+H]⁺ = 889.5 ± 0.5 Da (or full-length thymosin β4 multivalent charge envelope where applicable).
- Residual Moisture (Karl Fischer Titration)
- ≤ 2.5% water content.
- Endotoxin Screening (LAL Assay)
- < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
- Sterility Validation
- USP <71> compliant (no observed microbial proliferation).
- Physical Characterization
- Dense, white to off-white lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at −20°C to −80°C, protected from atmospheric moisture and direct light.
Solubility. Soluble in sterile laboratory-grade deionized water (≥ 10 mg/mL) or sterile Phosphate-Buffered Saline (PBS, pH 7.4). Use degassed buffers to minimize oxidative modification of methionine residues in full-length variants.
- Handling & Aliquoting: Prepare concentrated stock solutions freshly or aliquot into single-use fractions at −20°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Structural Origin & Classification: Synthetic active fragment of the β-thymosin actin-binding peptide thymosin β4, corresponding to the N-terminal actin-binding motif LKKTETQ (residues 17-23).
Actin Dynamics & Cytoskeletal Remodeling: Investigated in vitro for stoichiometric sequestration of globular actin (G-actin) and regulation of actin filament turnover, which underpin directed cell migration and cytoskeletal reorganization in cell culture models.
Cell Motility & Extracellular Matrix Signaling: Documented in endothelial, fibroblast, and myoblast assays for upregulation of matrix metalloproteinases (MMP-2, MMP-9) and promotion of chemotactic cell migration in wound-relevant in vitro models.
Technical Laboratory FAQs
What is the sequence and classification of TB-500?
TB-500 is the synthetic N-terminal active fragment of thymosin β4, with the acetylated sequence Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln (LKKTETQ) and an observed monoisotopic [M+H]⁺ of 889.5 Da.
How is TB-500 evaluated in actin-dynamics and cell-migration assays in vitro?
Preclinical literature examines TB-500 for G-actin sequestration, actin filament turnover, MMP expression, and directed cell migration in endothelial, fibroblast, and myoblast cell culture models.
What solvent matrix is recommended for preparing in vitro TB-500 stock solutions?
Sterile deoxygenated deionized water or sterile PBS (pH 7.4) provides rapid dissolution at 10 mg/mL or greater; degassed buffers minimize oxidative modification during downstream cell culture assays.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.