NAD+
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β-Nicotinamide adenine dinucleotide (NAD+), high-purity reference standard for in vitro laboratory research and analysis.
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$62.96
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Quantity
Out of stock
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Certificate of Analysis & Specifications
Documentation will be published once this lot completes third-party analytical release.
Released COA Library
Published Certificates of Analysis for NAD+. All released analytical records are preserved here. Pending and in-testing lots are reachable only through the QR printed on the vial.
1000mg1 released lot
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Lot KRSNAD-1000-260827-01
Tested Sep 3, 2026 · KMD Analytical · HPLC 99.48% · Assay 999.83
Related research materials
Research Data
Classification note. This catalog entry is presented as a research compound, not a research peptide. The label is for product classification and search clarity; it is not a recommendation for use.
Chemical & Structural Identifiers
- Chemical Name (IUPAC)
- Pyridine-adenine dinucleotide consisting of a nicotinamide mononucleotide (NMN) moiety connected to an adenosine monophosphate (AMP) core via a high-energy phosphoanhydride (pyrophosphate) linkage.
- CAS Registry Number
- 53-84-9 (Free zwitterion / inner salt); 20111-18-6 (Monosodium salt)
- PubChem CID
- 5892
- Molecular Formula
- C21H27N7O14P2
- Molecular Weight
- 663.11 Da (663.43 g/mol average; monosodium salt: 685.41 g/mol)
- SMILES
- C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C4N=CN=C5N)O)O)O)O)C(=O)N
- InChIKey
- BAWFJGJZGIEFAR-NNYOXOHSSA-N
- Salt / Counter-Ion
- Supplied as a high-purity crystalline free acid, zwitterionic inner salt, or lyophilized sodium (Na+) salt solid.
- Synonyms
- NAD+, NAD, β-NAD+, Coenzyme I, Nadide, Diphosphopyridine nucleotide (DPN), Oxidized DPN.
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.5% by area normalization under UV detection at λ = 260 nm (adenine and pyridinium nucleotide absorption maximum).
- Identity Confirmation (RP-HPLC RT + UV vs. reference)
- Main peak retention time and UV spectrum conform to the reference standard.
- Physical Characterization
- Dense, white to off-white or pale yellowish lyophilized crystalline powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light (highly hygroscopic).
Solubility. Freely soluble in sterile laboratory-grade deionized water (≥ 50 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.2–7.4, ≥ 30 mg/mL) for in vitro assay application.
- Hydrolytic Stability & pH Control: The quaternary N-glycosidic bond linking the nicotinamide ring to the ribose core is susceptible to base-catalyzed cleavage into free nicotinamide and ADP-ribose at alkaline pH > 8.0; prepare stock solutions in neutral to slightly acidic aqueous buffers (pH 6.0--7.2), avoid prolonged thermal exposure, and store single-use aliquots at -80°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Primary Biochemical Function & Classification: Central metabolic pyridine nucleotide coenzyme that functions both as a reversible electron carrier in cellular redox biochemistry (NAD+/NADH couple) and as an obligate, rate-limiting co-substrate for non-redox NAD+-consuming signaling enzymes.
Cellular Pathways Investigated: Evaluated across diverse in vitro cellular systems (including primary cortical neurons, cardiomyocytes, and skeletal muscle lines):
References (3)
- Imai, S., & Guarente, L. (2014). NAD+ and sirtuins in aging and disease. Trends in Cell Biology, 24(8), 464–471.
- Verdin, E. (2015). NAD+ in aging, metabolism, and neurodegeneration. Science, 350(6265), 1208–1213.
- Cambronne, X. A., et al. (2016). Biosensor reveals multiple sources for mitochondrial NAD+. Science, 352(6292), 1474–1477.
Technical Laboratory FAQs
How does the biochemical role of NAD+ in redox reactions differ from its function as a signaling co-substrate?
In redox metabolism (glycolysis, TCA cycle), NAD+ undergoes reversible two-electron reduction to NADH without net consumption of the dinucleotide molecule. In regulatory signaling (Sirtuins, PARPs, CD38), NAD+ is irreversibly consumed, cleaving the N-glycosidic linkage to release free nicotinamide and ADP-ribosyl derivatives.
Why is maintaining a neutral-to-slightly-acidic pH critical when handling NAD+ solutions?
The positively charged nicotinamide ring destabilizes the N-glycosidic bond under alkaline conditions (pH > 8.0), resulting in rapid non-enzymatic hydrolysis to ADP-ribose and nicotinamide, which can compromise analytical measurements and cell assay integrity.
What solvent conditions are recommended for preparing in vitro NAD+ stock solutions?
Sterile deoxygenated deionized water or sterile neutral PBS (pH 7.2–7.4) provides rapid dissolution at concentrations exceeding 30 mg/mL, establishing a stable working standard for enzymatic and cell-based metabolic assays.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.