BPC-157 + TB-500 Blend
Lot #KRSTBB-10-260908-01From $57.55to $517.12
Research blend of thymosin beta-4 fragment (TB-500) and BPC-157, lyophilized reference standard for in vitro laboratory research.
Price (/ pack)
$479.63
Total
10 total vials
$479.63
✓ Total Savings: You save $159.87 (25% total savings vs regular price)
Quantity
In Stock · 2 Units Available
This strength’s Certificate of Analysis isn’t published yet — add to cart will be enabled once the lab report is available.
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Certificate of Analysis & Specifications
Documentation will be published once this lot completes third-party analytical release.
Released COA Library
Published Certificates of Analysis for BPC-157 + TB-500 Blend. All released analytical records are preserved here. Pending and in-testing lots are reachable only through the QR printed on the vial.
No released certificate for this material yet
Reports appear here once a batch has completed third-party analytical release.
Related research materials
Research Data
Chemical & Structural Identifiers
- Formulation
- Binary co-lyophilized multi-peptide laboratory reference complex (dual-action standard combining Pentadecapeptide BPC-157 and Thymosin β4 / TB-500 active fragment).
- Salt / Counter-Ion
- Supplied as a stoichiometric co-lyophilized composite standard in stable acetate (CH3COO-) or trifluoroacetate (CF3COO-) salt forms.
Constituent Peptides
BPC-157
Chemical Name: L-valyl-L-alanyl-L-aspartyl-L-alanyl-L-glycyl-L-leucyl-L-valyl-L-prolyl-L-prolyl-L-prolylglycyl-L-lysyl-L-prolyl-L-alanyl-L-aspartic acid · Primary Sequence: H-Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val-OH · CAS Registry Number: 137525-51-0 · PubChem CID: 9941957 · Molecular Formula: C62H98N16O22 · Monoisotopic Molecular Weight: 1418.70 Da (1419.55 g/mol average)
TB-500 / Thymosin β4 Active Fragment
Chemical Name: N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-threonyl-L-glutamyl-L-threonyl-L-glutamine (Ac-LKKTETQ, residues 17–23) or full-length synthetic 43-amino acid human Thymosin β4 · Primary Sequence: Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH (7-mer active actin-binding domain) or Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES (full 43-mer) · CAS Registry Number: 885340-08-9 (active fragment) / 77591-33-4 (full sequence) · Molecular Formula: C38H68N10O14 (7-mer fragment) / C212H350N56O78S (full 43-mer) · Monoisotopic Molecular Weight: 888.49 Da (889.01 g/mol fragment) / 4960.49 Da (4963.50 g/mol full 43-mer)
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- Individual peptide purities ≥ 98.5% (composite purity ≥ 98.0%) via baseline-resolved dual-peak gradient elution under UV detection at λ = 214 nm and 220 nm.
- BPC-157 identity
- Conforms to reference by retention time and UV spectrum.
- TB-500 fragment identity
- Conforms to reference by retention time and UV spectrum.
- Physical Characterization
- Dense, white to off-white lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light.
Solubility. Soluble in sterile laboratory-grade deionized water (≥ 15 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 10 mg/mL) for in vitro assay application.
- Handling & Methionine Oxidation Controls: Full-length Tβ4 contains an internal methionine residue (Met6) susceptible to sulfoxide oxidation; prepare working solutions using degassed neutral buffers (pH 7.0--7.4), avoid high-shear vortexing, and store single-use aliquots at -20°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Dual-Pathway Synergy Model: Investigated in cell culture and tissue explant models to examine non-overlapping mechanisms of cellular motility, cytoskeletal restructuring, and focal adhesion dynamics.
BPC-157 Axis (FAK-Paxillin & Gene Transcription): Evaluated in fibroblasts, tendon explants, and endothelial cell lines to monitor the phosphorylation of Focal Adhesion Kinase (FAK) and paxillin, transcriptional activation of Early Growth Response-1 (Egr-1), and modulation of the VEGFR2 signaling cascade.
TB-500 Axis (Actin Dynamics & Chemotaxis): Evaluated in endothelial and myoblast scratch wound assays for 1:1 stoichiometric sequestration of globular actin (G-actin), regulation of actin filament turnover, upregulation of Matrix Metalloproteinases (MMP-2, MMP-9), and stimulation of directed cell migration.
References (3)
- Chang, C. H., et al. (2011). Pentadecapeptide BPC 157 enhances FAK and paxillin phosphorylation in in-vitro cell migration assays. Biochemical and Biophysical Research Communications, 404(4), 1015–1020.
- Philp, D., et al. (2003). Thymosin beta 4 and its active peptide fragment promote dermal cell migration and matrix deposition in vitro. Journal of Cell Science, 116(15), 3229–3236.
- Sikiric, P., et al. (2018). Stable gastric pentadecapeptide BPC 157, vascular endothelial growth factor, and cellular signaling cascades. Current Pharmaceutical Design, 24(18), 1956–1966.
Technical Laboratory FAQs
Why are BPC-157 and TB-500 combined in cell motility and extracellular matrix (ECM) assays?
The co-formulation allows researchers to investigate two complementary cellular repair pathways simultaneously: BPC-157 primarily modulates FAK/paxillin focal adhesion signaling and Egr-1 expression, while TB-500 directly sequesters G-actin to drive cytoskeletal remodeling and chemotactic cell migration.
How are BPC-157 and TB-500 resolved analytically in a single composite sample?
Reverse-phase HPLC (RP-HPLC) achieves chromatographic separation between the 15-amino-acid BPC-157 and the TB-500 peptide (fragment or full sequence), with each peak identified by retention time and UV spectral comparison against the reference standard.
What reconstitution parameters maintain stability for in vitro TB-500/BPC-157 stocks?
Sterile deoxygenated deionized water or sterile PBS (pH 7.4) provides rapid dissolution at concentrations ≥ 10 mg/mL. Using degassed buffers prevents oxidative modification of methionine residues in full-length TB-500 during downstream cell culture assays.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.