Quantity
Price
$40.00
100 available
This strength’s Certificate of Analysis isn’t published yet — add to cart will be enabled once the lab report is available.
Thymalin
From $40.00to $300.00
For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.
This item is available only through approved account access.
Product Specifications
- Lot Number
- —
- Purity
- —
- Storage Temp
- 36°F to 46°F
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.
Documentation
Certificates of Analysis are supplied with each product lot where applicable.
Certificate of Analysis (COA)
Documentation will be available once product lots are received and tested.
All products currently listed on this site are for research purposes only.
COA Library
Published Certificates of Analysis for Thymalin. New deliveries are added as their batches are released.
No released COA for this strength yet
Reports appear here once a batch for this strength has been released.
Research Data
Chemical & Structural Identifiers
- Chemical Name (IUPAC)
- Natural low-molecular-weight polypeptide complex extracted from the thymus gland of young cattle (Bos taurus).
- CAS Registry Number
- 63958-90-7
- PubChem CID
- Biological Extract Complex ID / BioChemEntity.
- Molecular Weight
- Heterogeneous peptide extract with molecular weights predominantly ≤ 10.0 kDa (median bioactive fraction: 300--5000 Da).
- Salt / Counter-Ion
- Supplied as a high-purity, sterile-filtered, lyophilized natural polypeptide complex in a stable zwitterionic solid matrix.
- Synonyms
- Thymalin, Timalin, Thymalinum, Thymus extract polypeptide complex, Natural Thymus Bioregulator Complex.
- Primary Structural Composition: Defined biological pool of endogenous thymic peptides and oligopeptides (predominantly containing regulatory thymic fractions, including endogenous Glu-Trp and associated low-molecular-weight peptide chains).
Analytical & COA Specifications
- Analytical Characterization (RP-HPLC / Size-Exclusion SEC)
- Chromatographic peptide fingerprint profile matching standard natural thymus reference spectra; total peptide content ≥ 90.0% (quantified by total nitrogen/Kjeldahl or bicinchoninic acid assay).
- Identity Confirmation (Multi-component ESI-MS / SDS-PAGE)
- Multi-peak molecular mass distribution within the 0.3--5.0 kDa range; characteristic electrophoretic migration pattern matching validated bovine thymus polypeptide standards.
- Residual Moisture (Karl Fischer Titration)
- ≤ 3.0% composite water content.
- Endotoxin Screening (LAL Assay)
- < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
- Sterility Validation
- USP <71> compliant (no observed microbial proliferation in fluid thioglycollate or soybean-casein digest broths).
- Physical Characterization
- Dense, white to off-white or pale cream lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light.
Solubility. Soluble in sterile laboratory-grade deionized water (≥ 10 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 5 mg/mL) for in vitro assay application.
- Multi-Peptide Solution Handling: As a natural multi-peptide biological complex, avoid excessive foaming, high-shear vortexing, or prolonged exposure to ambient heat (≥ 37°C) prior to cell culture dosing; aliquot into single-use fractions at -20°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Primary Biochemical Classification & Origin: Foundational natural peptide bioregulator extracted from bovine thymus tissue, developed within the Khavinson bioregulator framework. Served as the natural polypeptide prototype from which targeted synthetic short peptides (such as the dipeptide Thymagen/Thymogen) were originally identified and synthesized.
Cellular Pathways Investigated: Evaluated across in vitro primary thymocyte cultures, splenocytes, peripheral blood mononuclear cells (PBMCs), and bone marrow precursor lines to quantify:
References (3)
- Morozov, V. G., & Khavinson, V. K. (1997). Natural and synthetic thymic peptides. International Journal of Immunopharmacology, 19(9-10), 501–505.
- Khavinson, V. K., et al. (2014). Peptide regulation of gene expression and protein synthesis in cell-free and cellular models. Bulletin of Experimental Biology and Medicine, 157(1), 142–146.
- Kuznik, B. I., et al. (2011). Thymalin and Epithalamin in the regulation of the immune system and hemostasis: molecular mechanisms and cellular perspectives in vitro. Advances in Gerontology, 1(4), 291–297.
Technical Laboratory FAQs
What distinguishes natural Thymalin from synthetic Thymagen (Thymogen) in vitro?
Thymalin is a natural multi-peptide extract derived directly from bovine thymus tissue containing a wide spectrum of endogenous low-molecular-weight peptides (≤ 10 kDa), whereas Thymagen is a single, pure synthetic dipeptide (Glu-Trp) isolated as the minimal active immunomodulatory motif from the natural extract.
Which cellular immunological markers are primarily monitored in vitro following Thymalin exposure?
Assays routinely quantify the induction and surface density of T-lymphocyte differentiation clusters, specifically CD3+, CD4+ helper, and CD8+ cytotoxic markers, alongside the secretion kinetics of IL-2 and IFN-γ in cell culture supernatants.
What reconstitution vehicle is recommended for preparing in vitro Thymalin working stocks?
Sterile laboratory-grade deionized water or sterile PBS (pH 7.4) provides complete dissolution at concentrations ≥ 5 mg/mL, creating an isotonic carrier solution suitable for downstream cell viability, flow cytometry, and cytokine profiling assays.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.