TB/BP BLEND

Pack

Quantity

Price

$60.00

10% off$66.67

100 available

This strength’s Certificate of Analysis isn’t published yet — add to cart will be enabled once the lab report is available.

TB-500/BPC-157 Blend

Name: TB-500/BPC-157 Blend
Brand: KAM Research
Availability: InStock

From $60.00to $450.00

For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.

This item is available only through approved account access.

Product Specifications

Lot Number: —
Purity: —
Storage Temp: 36°F to 46°F

For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.

Documentation

Certificates of Analysis are supplied with each product lot where applicable.

Certificate of Analysis (COA)

Documentation will be available once product lots are received and tested.

All products currently listed on this site are for research purposes only.

COA Library

Published Certificates of Analysis for TB-500/BPC-157 Blend. New deliveries are added as their batches are released.

No released COA for this strength yet

Reports appear here once a batch for this strength has been released.

Research Data

Chemical & Structural Identifiers

Formulation: Binary co-lyophilized multi-peptide laboratory reference complex (dual-action standard combining Pentadecapeptide BPC-157 and Thymosin β₄ / TB-500 active fragment).
Salt / Counter-Ion: Supplied as a stoichiometric co-lyophilized composite standard in stable acetate (CH₃COO^-) or trifluoroacetate (CF₃COO^-) salt forms.

Constituent Peptides

BPC-157

Chemical Name: L-valyl-L-alanyl-L-aspartyl-L-alanyl-L-glycyl-L-leucyl-L-valyl-L-prolyl-L-prolyl-L-prolylglycyl-L-lysyl-L-prolyl-L-alanyl-L-aspartic acid · Primary Sequence: H-Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val-OH · CAS Registry Number: 137525-51-0 · PubChem CID: 9941957 · Molecular Formula: C₆₂H₉₈N₁₆O₂₂ · Monoisotopic Molecular Weight: 1418.70 Da (1419.55 g/mol average)

TB-500 / Thymosin β₄ Active Fragment

Chemical Name: N-acetyl-L-leucyl-L-lysyl-L-lysyl-L-threonyl-L-glutamyl-L-threonyl-L-glutamine (Ac-LKKTETQ, residues 17–23) or full-length synthetic 43-amino acid human Thymosin β₄ · Primary Sequence: Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH (7-mer active actin-binding domain) or Ac-SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES (full 43-mer) · CAS Registry Number: 885340-08-9 (active fragment) / 77591-33-4 (full sequence) · Molecular Formula: C₃₈H₆₈N₁₀O₁₄ (7-mer fragment) / C₂₁₂H₃₅₀N₅₆O₇₈S (full 43-mer) · Monoisotopic Molecular Weight: 888.49 Da (889.01 g/mol fragment) / 4960.49 Da (4963.50 g/mol full 43-mer)

Analytical & COA Specifications

Analytical Purity (RP-HPLC): Individual peptide purities ≥ 98.5% (composite purity ≥ 98.0%) via baseline-resolved dual-peak gradient elution under UV detection at λ = 214 nm and 220 nm.
BPC-157 observed: [M+H]⁺ = 1419.6 ± 0.5 Da
TB-500 fragment observed: [M+H]⁺ = 889.5 ± 0.5 Da (or full-length Tβ₄ multivalent charge envelope: [M+4H]⁴⁺ = 1241.9 Da, [M+5H]⁵⁺ = 993.7 Da)
Residual Moisture (Karl Fischer Titration): ≤ 2.5% composite water content.
Endotoxin Screening (LAL Assay): < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
Sterility Validation: USP <71> compliant (no observed microbial proliferation in fluid thioglycollate or soybean-casein digest broths).
Physical Characterization: Dense, white to off-white lyophilized solid cake/powder.

Handling & Stability

Storage. Store dry solid desiccated at -20^°C to -80^°C, protected from atmospheric moisture and direct light.

Solubility. Soluble in sterile laboratory-grade deionized water (≥ 15 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 10 mg/mL) for in vitro assay application.

Handling & Methionine Oxidation Controls: Full-length Tβ₄ contains an internal methionine residue (Met⁶) susceptible to sulfoxide oxidation; prepare working solutions using degassed neutral buffers (pH 7.0--7.4), avoid high-shear vortexing, and store single-use aliquots at -20^°C to eliminate degradation from repeated freeze-thaw cycles.

Mechanism & Literature

Dual-Pathway Synergy Model: Investigated in cell culture and tissue explant models to examine non-overlapping mechanisms of cellular motility, cytoskeletal restructuring, and focal adhesion dynamics.

BPC-157 Axis (FAK-Paxillin & Gene Transcription): Evaluated in fibroblasts, tendon explants, and endothelial cell lines to monitor the phosphorylation of Focal Adhesion Kinase (FAK) and paxillin, transcriptional activation of Early Growth Response-1 (Egr-1), and modulation of the VEGFR2 signaling cascade.

TB-500 Axis (Actin Dynamics & Chemotaxis): Evaluated in endothelial and myoblast scratch wound assays for 1:1 stoichiometric sequestration of globular actin (G-actin), regulation of actin filament turnover, upregulation of Matrix Metalloproteinases (MMP-2, MMP-9), and stimulation of directed cell migration.

References (3)

Chang, C. H., et al. (2011). Pentadecapeptide BPC 157 enhances FAK and paxillin phosphorylation in in-vitro cell migration assays. Biochemical and Biophysical Research Communications, 404(4), 1015–1020.
Philp, D., et al. (2003). Thymosin beta 4 and its active peptide fragment promote dermal cell migration and matrix deposition in vitro. Journal of Cell Science, 116(15), 3229–3236.
Sikiric, P., et al. (2018). Stable gastric pentadecapeptide BPC 157, vascular endothelial growth factor, and cellular signaling cascades. Current Pharmaceutical Design, 24(18), 1956–1966.

Technical Laboratory FAQs

Why are BPC-157 and TB-500 combined in cell motility and extracellular matrix (ECM) assays?

The co-formulation allows researchers to investigate two complementary cellular repair pathways simultaneously: BPC-157 primarily modulates FAK/paxillin focal adhesion signaling and Egr-1 expression, while TB-500 directly sequesters G-actin to drive cytoskeletal remodeling and chemotactic cell migration.

How are BPC-157 and TB-500 resolved analytically in a single composite sample?

Reverse-phase HPLC (RP-HPLC) achieves chromatographic separation between the 15-amino-acid BPC-157 and the TB-500 peptide (fragment or full sequence), with electrospray ionization mass spectrometry (ESI-MS) confirming individual molecular mass signatures (1419.6 Da and 889.5 Da / 4963.5 Da, respectively).

What reconstitution parameters maintain stability for in vitro TB-500/BPC-157 stocks?

Sterile deoxygenated deionized water or sterile PBS (pH 7.4) provides rapid dissolution at concentrations ≥ 10 mg/mL. Using degassed buffers prevents oxidative modification of methionine residues in full-length TB-500 during downstream cell culture assays.

For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.