Quantity
Price
$33.00
100 available
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Snap-8
From $33.00to $247.50
For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.
This item is available only through approved account access.
Product Specifications
- Lot Number
- —
- Purity
- —
- Storage Temp
- 36°F to 46°F
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.
Documentation
Certificates of Analysis are supplied with each product lot where applicable.
Certificate of Analysis (COA)
Documentation will be available once product lots are received and tested.
All products currently listed on this site are for research purposes only.
COA Library
Published Certificates of Analysis for Snap-8. New deliveries are added as their batches are released.
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Reports appear here once a batch for this strength has been released.
Research Data
Chemical & Structural Identifiers
- Chemical Name (IUPAC)
- N-acetyl-L-α-glutamyl-L-α-glutamyl-L-methionyl-L-glutaminyl-L-arginyl-L-arginyl-L-alanyl-L-aspartamide ((2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-carbamimidamido-2-[[(2S)-5-carbamimidamido-2-[[(2S)-4-carbamoyl-2-[[(2S)-2-[[(2S)-2-acetamido-4-carboxybutanoyl]amino]-4-carboxybutanoyl]amino]-4-methylsulfanylbutanoyl]amino]pentanoyl]amino]pentanoyl]amino]propanoyl]amino]-4-amino-4-oxobutanoic acid)
- CAS Registry Number
- 868844-74-0
- PubChem CID
- 16133827
- Molecular Formula
- C41H70N16O16S
- Molecular Weight
- 1074.48 Da (1075.16 g/mol average)
- Sequence
- Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2 (Single-letter: Ac-EEMQRRAD-NH2)
- SMILES
- CC(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(=O)O)C(=O)N
- InChIKey
- UBFGGOMVQLKTSW-NNAZSAHJSA-N
- Salt / Counter-Ion
- Supplied as a high-purity lyophilized acetate (CH3COO-) or trifluoroacetate (CF3COO-) salt.
- Synonyms
- SNAP-8, Snap-8, Acetyl Octapeptide-3, Acetyl Octapeptide-1, Acetyl Glutamyl Heptapeptide-3, Octapeptide-3, Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.5% by area normalization under UV detection at λ = 214 nm and 220 nm.
- Identity Confirmation (ESI-MS / MALDI-TOF)
- Theoretical mass [M+H]+ = 1075.5 Da; observed mass-to-charge ratio m/z = 1075.5 ± 0.5 Da (with confirmed divalent ion [M+2H]2+ = 538.3 Da).
- Residual Moisture (Karl Fischer Titration)
- ≤ 2.0% water content.
- Endotoxin Screening (LAL Assay)
- < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
- Sterility Validation
- USP <71> compliant (no observed microbial proliferation in fluid thioglycollate or soybean-casein digest broths).
- Physical Characterization
- Dense, white to off-white lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light.
Solubility. Highly hydrophilic; freely soluble in sterile laboratory-grade deionized water (≥ 20 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 15 mg/mL) for in vitro assay application.
- Methionine Oxidation & Handling: Contains an internal methionine residue (Met3) susceptible to sulfoxide oxidation under aerobic conditions; prepare working solutions using degassed buffers, avoid strong oxidizers or excessive vortexing, and store single-use aliquots at -20°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Synthetic biomimetic octapeptide engineered as an elongated analogue of the N-terminal domain of SNAP-25 (Synaptosomal-Associated Protein 25). Functions as a competitive inhibitor of SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) complex assembly.
SNARE Complex Destabilization Kinetics: Evaluated in cell-free biochemical assays and chromaffin/neuronal cell cultures:
Structural Distinction from Argireline: An extension of the hexapeptide Argireline (Ac-EEMQRR-NH2) by two additional C-terminal residues (Ala7-Asp8). This elongation enhances steric hindrance within the SNARE bundle, providing increased potency in cellular catecholamine and acetylcholine release inhibition assays.
References (3)
- Blanes-Mira, C., et al. (2002). A synthetic hexapeptide (Argireline) with antiwrinkle activity. International Journal of Cosmetic Science, 24(5), 303–310.
- Ruiz, M. A., et al. (2007). In vitro and in vivo characterization of an anti-aging octapeptide (SNAP-8) targeting SNARE complex formation. International Journal of Cosmetic Science, 29(6), 461–468.
- Gutierrez, L. M., et al. (1997). A peptide that mimics the carboxy-terminal domain of SNAP-25 blocks calcium-dependent exocytosis in chromaffin cells. FEBS Letters, 408(3), 321–326.
Technical Laboratory FAQs
What structural difference distinguishes SNAP-8 from Argireline in vitro?
SNAP-8 (Ac-EEMQRRAD-NH2) is an octapeptide containing two additional amino acids (Ala7-Asp8) at the C-terminus compared to the hexapeptide Argireline (Ac-EEMQRR-NH2), providing greater conformational stability and steric inhibition of SNARE complex formation in biochemical assays.
How does SNAP-8 disrupt vesicular neurotransmitter release in cell culture models?
SNAP-8 mimics the N-terminal domain of SNAP-25, competing for incorporation into the ternary SNARE complex with syntaxin and synaptobrevin. This destabilizes the coiled-coil core complex, blocking vesicle fusion and preventing calcium-triggered exocytosis of acetylcholine.
What reconstitution vehicle is recommended for preparing in vitro SNAP-8 working stocks?
Sterile deionized water or sterile PBS (pH 7.4) provides rapid dissolution at concentrations ≥ 15 mg/mL. Preparing working stocks with degassed buffers protects the Met3 thioether group against air oxidation during long-term experimental series.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.