PE-22-28
From $37.75to $314.62
Peptide PE-22-28, lyophilized reference standard for in vitro laboratory research.
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Price
$37.76
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Quantity
In Stock · 100 Units Available
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Certificate of Analysis & Specifications
Documentation will be published once this lot completes third-party analytical release.
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Research Data
Chemical & Structural Identifiers
![2D chemical structure of L-serylglycyl-L-$\alpha$-aspartyl-L-threonyl-L-seryl-L-prolyl-L-arginine ((2S)-2-[[(2S)-1-[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[2-[[(2S)-2-amino-3-hydroxypropanoyl]amino]acetyl]amino]-3-carboxypropanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]pyrrolidine-2-carbonyl]amino]-5-guanidinopentanoic acid)](/structures/pe-22-28.svg)
- Chemical Name (IUPAC)
- L-serylglycyl-L-α-aspartyl-L-threonyl-L-seryl-L-prolyl-L-arginine ((2S)-2-[[(2S)-1-[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[2-[[(2S)-2-amino-3-hydroxypropanoyl]amino]acetyl]amino]-3-carboxypropanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]pyrrolidine-2-carbonyl]amino]-5-guanidinopentanoic acid)
- CAS Registry Number
- 1616422-96-8
- PubChem CID
- 137700202
- Molecular Formula
- C27H46N10O13
- Molecular Weight
- 718.32 Da (718.72 g/mol average)
- Sequence
- H-Ser-Gly-Asp-Thr-Ser-Pro-Arg-OH (Single-letter: SGDTSPR)
- SMILES
- CC(C(C(=O)NC(CO)C(=O)N1CCCC1C(=O)NC(CCCNC(=N)N)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)CNC(=O)C(CO)N)O
- InChIKey
- AQNZQZJSHGJWQO-ZEXUOBIOSA-N
- Salt / Counter-Ion
- Supplied as a high-purity lyophilized acetate (CH3COO-) or trifluoroacetate (CF3COO-) salt.
- Synonyms
- PE-22-28, PE 22-28, Mini-Spadin, Sortilin Propeptide Fragment (22-28), SGDTSPR, TREK-1 Channel Inhibitor Peptide
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.5% by area normalization under UV detection at λ = 214 nm and 220 nm.
- Identity Confirmation (RP-HPLC RT + UV vs. reference)
- Main peak retention time and UV spectrum conform to the reference standard.
- Physical Characterization
- Dense, white to off-white lyophilized crystalline cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light.
Solubility. Highly hydrophilic; freely soluble in sterile laboratory-grade deionized water (≥ 20 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 15 mg/mL) for in vitro assay delivery.
- Aliquoting & Solution Stability: Contains serine and threonine hydroxyl side chains alongside a flexible C-terminal proline-arginine motif; prepare working stock solutions freshly or aliquot into single-use fractions at -20°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Functions as a potent, selective antagonist of the TREK-1 two-pore domain potassium channel (K2P2.1 / KCNK2) with sub-nanomolar affinity (IC50 ≈ 0.12 nM), demonstrating significantly higher stability and potency than parent Spadin.
Biochemical Origin & Structure-Activity Optimization: Synthetic heptapeptide developed as a shortened, bio-stable derivative of Spadin (PE 12-28), a 17-amino acid peptide derived from the prodomain of sortilin. PE-22-28 represents the minimal functional pharmacophore capable of high-affinity blockade of two-pore domain potassium channels.
Cellular Pathways Investigated: Evaluated across in vitro primary hippocampal neurons, cortical cultures, and TREK-1 transfected cell lines (HEK293, CHO) to measure:
References (3)
- Veyssiere, J., et al. (2020). PE-22-28, a Spadin-derived peptide, displays fast-acting antidepressant properties and enhances neurogenesis in vitro and in vivo. Neuropharmacology, 162, 107812.
- Borsotto, M., et al. (2015). Targeting the TREK-1 potassium channel with Spadin and derived peptides: A new horizon for depression modeling. Channels, 9(5), 260–267.
- Moha ou Maati, H., et al. (2012). Spadin as a new antidepressant: from the discovery to the development of shortened analogs. Frontiers in Pharmacology, 3, 172.
Technical Laboratory FAQs
How does PE-22-28 relate structurally to full-length Spadin (PE 12-28)?
PE-22-28 is a truncated synthetic heptapeptide containing the C-terminal residues 22 to 28 (Ser-Gly-Asp-Thr-Ser-Pro-Arg) of the sortilin propeptide, retaining full TREK-1 blocking activity with improved aqueous stability and resistance to enzymatic degradation.
What is the primary molecular target of PE-22-28 in electrophysiology assays?
PE-22-28 selectively inhibits the two-pore domain potassium channel TREK-1 (K2P2.1 / KCNK2), blocking outward leak potassium currents at sub-nanomolar concentrations in cellular electrophysiology models.
What reconstitution solvent is recommended for preparing in vitro PE-22-28 stocks?
Sterile deionized water or sterile PBS (pH 7.4) provides rapid dissolution at concentrations ≥ 15 mg/mL, creating an isotonic carrier standard for patch-clamp electrophysiology and neuronal cell culture assays.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.