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$64.00
100 available
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KLOW
From $64.00to $480.00
For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.
This item is available only through approved account access.
Product Specifications
- Lot Number
- —
- Purity
- —
- Storage Temp
- 36°F to 46°F
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.
Documentation
Certificates of Analysis are supplied with each product lot where applicable.
Certificate of Analysis (COA)
Documentation will be available once product lots are received and tested.
All products currently listed on this site are for research purposes only.
COA Library
Published Certificates of Analysis for KLOW. New deliveries are added as their batches are released.
No released COA for this strength yet
Reports appear here once a batch for this strength has been released.
Research Data
Chemical & Structural Identifiers
- Formulation
- Quaternary co-lyophilized peptide reference complex (Multi-target laboratory standard comprising KPV Tripeptide, Copper Tripeptide-1 [GHK-Cu], Pentadecapeptide BPC-157, and Thymosin β4 active fragment TB-500).
- Salt / Counter-Ion
- Supplied as a stoichiometric co-lyophilized composite standard in stable acetate (CH3COO-) and cupric chelate salt forms.
Constituent Peptides
KPV
H-Lys-Pro-Val-OH (Lys-Pro-Val) · CAS: 67727-97-3 · Formula: C16H31N5O4 · MW: 357.45 g/mol
GHK-Cu
H-Gly-His-Lys-OH·Cu2+ · CAS: 49557-75-7 · Formula: C14H22CuN6O4 · MW: 401.91 g/mol
BPC-157
H-Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val-OH · CAS: 137525-51-0 · Formula: C62H98N16O22 · MW: 1419.55 g/mol
TB-500 Fragment
Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln-OH (Ac-LKKTETQ) · CAS: 77591-33-4 / 885340-08-9 · Formula: C38H68N10O14 · MW: 889.01 g/mol
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- Individual constituent purities ≥ 98.0% via baseline-resolved multi-gradient elution under UV detection at λ = 214 nm and 254 nm.
- KPV observed
- [M+H]+ = 358.2 ± 0.4 Da
- GHK-Cu observed
- [M+H]+ = 402.1 ± 0.5 Da (with copper natural abundance isotopic distribution)
- TB-500 observed
- [M+H]+ = 889.5 ± 0.5 Da
- BPC-157 observed
- [M+H]+ = 1419.6 ± 0.5 Da
- Residual Moisture (Karl Fischer Titration)
- ≤ 3.0% composite water content.
- Endotoxin Screening (LAL Assay)
- < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
- Sterility Validation
- USP <71> compliant (no observed microbial proliferation in fluid thioglycollate or soybean-casein digest broths).
- Physical Characterization
- Distinctive light cyan to royal blue lyophilized cake/powder (governed by the coordination geometry of the Cu2+ complex).
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, sealed under an inert gas matrix and protected from light.
Solubility. Soluble in sterile laboratory-grade deionized water (≥ 15 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 10 mg/mL) for in vitro assay application.
- Handling & Buffer Compatibility: Maintain working solutions at neutral pH (pH 6.8--7.4). *Do not* introduce strong metal chelators (e.g., EDTA, EGTA) or reducing agents (e.g., DTT, β-mercaptoethanol) into stock buffers, as they strip the coordinated copper ion or disrupt peptide stability. Aliquot into single-use fractions to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Biochemical Research Classification: Quaternary multi-target peptide matrix designed for concurrent in vitro investigation of nuclear factor signaling, extracellular matrix (ECM) synthesis, focal adhesion dynamics, and cytoskeletal actin motility.
References (4)
- Luger, T. A., et al. (2003). The C-terminal tripeptide of alpha-MSH (KPV) inhibits NF-kappaB activation in vitro. Annals of the New York Academy of Sciences, 994(1), 133–140.
- Pickart, L., et al. (2018). GHK peptide as a natural modulator of multiple cellular pathways in gene expression profiling. Biomolecules, 8(4), 166.
- Chang, C. H., et al. (2011). Pentadecapeptide BPC 157 enhances FAK and paxillin phosphorylation in in-vitro cell migration assays. Biochemical and Biophysical Research Communications, 404(4), 1015–1020.
- Philp, D., et al. (2003). Thymosin beta 4 and its active peptide fragment promote dermal cell migration and matrix deposition in vitro. Journal of Cell Science, 116(15), 3229–3236.
Technical Laboratory FAQs
What constituent molecules form the KLOW multi-peptide reference standard?
The KLOW standard is a four-part co-lyophilized complex consisting of KPV (α-MSH C-terminal tripeptide), Copper Tripeptide-1 (GHK-Cu), Pentadecapeptide BPC-157, and the active actin-binding fragment of Thymosin β4 (TB-500).
How does KLOW differ functionally from the GLOW peptide blend in vitro?
KLOW incorporates the tripeptide KPV (Lys-Pro-Val), enabling researchers to concurrently examine NF-κB nuclear signaling modulation alongside the ECM deposition, FAK phosphorylation, and actin sequestering pathways evaluated by the GLOW trio.
How are all four components resolved analytically in a single composite vial?
RP-HPLC baseline separation resolves four distinct chromatographic peaks, confirmed by electrospray ionization mass spectrometry (ESI-MS) by tracking specific m/z masses: KPV (358.2 Da), GHK-Cu (402.1 Da), TB-500 (889.5 Da), and BPC-157 (1419.6 Da).
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.