Quantity
Price
$54.00
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Semax
From $54.00to $690.00
For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.
This item is available only through approved account access.
Product Specifications
- Lot Number
- —
- Purity
- —
- Storage Temp
- 36°F to 46°F
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.
Documentation
Certificates of Analysis are supplied with each product lot where applicable.
Certificate of Analysis (COA)
Documentation will be available once product lots are received and tested.
All products currently listed on this site are for research purposes only.
COA Library
Published Certificates of Analysis for Semax. New deliveries are added as their batches are released.
No released COA for this strength yet
Reports appear here once a batch for this strength has been released.
Research Data
Chemical & Structural Identifiers
![2D chemical structure of L-methionyl-L-$\alpha$-glutamyl-L-histidyl-L-phenylalanyl-L-prolyl-glycyl-L-proline ((2S)-1-[2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-phenylpropanoyl]pyrrolidine-2-carbonyl]amino]acetyl]pyrrolidine-2-carboxylic acid)](/structures/semax.svg)
- Chemical Name (IUPAC)
- L-methionyl-L-α-glutamyl-L-histidyl-L-phenylalanyl-L-prolyl-glycyl-L-proline ((2S)-1-[2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-4-carboxybutanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-3-phenylpropanoyl]pyrrolidine-2-carbonyl]amino]acetyl]pyrrolidine-2-carboxylic acid)
- CAS Registry Number
- 80714-61-0
- PubChem CID
- 122178
- Molecular Formula
- C37H51N9O10S
- Molecular Weight
- 813.35 Da (813.93 g/mol average)
- Sequence
- H-Met-Glu-His-Phe-Pro-Gly-Pro-OH (Single-letter: MEHFPGP)
- SMILES
- CSCCC(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CNC=N1)C(=O)NC(CC2=CC=CC=C2)C(=O)N3CCCC3C(=O)NCC(=O)N4CCCC4C(=O)O)N
- InChIKey
- OQGQHQXVOYOPND-UHFFFAOYSA-N
- Salt / Counter-Ion
- Supplied as a high-purity lyophilized acetate (CH3COO-) or trifluoroacetate (CF3COO-) salt.
- Synonyms
- Semax, ACTH(4-7)PGP, Heptapeptide Semax, Met-Glu-His-Phe-Pro-Gly-Pro.
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.5% by area normalization under UV detection at λ = 214 nm and 220 nm.
- Identity Confirmation (ESI-MS / MALDI-TOF)
- Theoretical mass [M+H]+ = 814.4 Da; observed mass-to-charge ratio m/z = 814.4 ± 0.4 Da (with confirmed divalent ion [M+2H]2+ = 407.7 Da).
- Residual Moisture (Karl Fischer Titration)
- ≤ 2.0% water content.
- Endotoxin Screening (LAL Assay)
- < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
- Sterility Validation
- USP <71> compliant (no observed microbial proliferation in fluid thioglycollate or soybean-casein digest broths).
- Physical Characterization
- Dense, white to off-white lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light.
Solubility. Highly hydrophilic; freely soluble in sterile laboratory-grade deionized water (≥ 20 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 15 mg/mL) for in vitro assay application.
- Methionine Oxidation & Terminal Stability: Contains an N-terminal methionine residue (Met1) susceptible to sulfoxide oxidation under aerobic conditions, balanced by a C-terminal -Pro-Gly-Pro (PGP) motif that resists carboxypeptidases; prepare working solutions using degassed buffers, avoid excessive vortexing, and store single-use aliquots at -20°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Structural Origin & Design Rationale: Synthetic regulatory heptapeptide engineered at the Institute of Molecular Genetics of the Russian Academy of Sciences. Synthesized by fusing the active adrenocorticotropic hormone core (ACTH4--7, Met-Glu-His-Phe) to the synthetic tripeptide Pro-Gly-Pro (PGP), eliminating hormonal steroidogenic activity while extending biological half-life and neurotrophic potency.
Cellular Pathways Investigated: Evaluated across in vitro neuronal models (such as primary cortical and hippocampal cultures, PC12 cells, and glial astrocytes) to assess:
References (3)
- Ashmarin, I. P., et al. (1997). Semax, an ACTH(4-10) analog with nootropic properties. Neuroscience and Behavioral Physiology, 27(4), 346–350.
- Gusev, E. I., et al. (2018). Mechanism of action of the peptide drug Semax in ischemic models. Zhurnal Nevrologii i Psikhiatrii Imeni S.S. Korsakova, 118(3), 32–37.
- Medvedeva, E. V., et al. (2014). The peptide Semax affects the expression of genes related to the immune and vascular systems in rat brain cells in vitro and in vivo. Molecular Genetics, Microbiology and Virology, 29(2), 71–78.
Technical Laboratory FAQs
How does Semax relate structurally to native adrenocorticotropic hormone (ACTH)?
Semax is an engineered peptide combining the central ACTH4--7 sequence (Met-Glu-His-Phe) with a stabilizing C-terminal Pro-Gly-Pro (PGP) tripeptide, preserving neuromodulatory actions without triggering adrenal steroidogenesis.
What cellular signaling pathways are triggered by Semax in neuronal cell assays in vitro?
In cell culture models, Semax stimulates the transcriptional expression of neurotrophic factors BDNF and NGF, modulates TrkB receptor signaling cascades, and downregulates pro-inflammatory gene expression under induced hypoxic and ischemic conditions.
What reconstitution conditions are recommended for preparing in vitro Semax stocks?
Sterile deionized water or sterile PBS (pH 7.4) provides rapid dissolution at concentrations ≥ 15 mg/mL. Preparing stocks with degassed buffers protects the Met1 thioether group against air oxidation during long-term experimental series.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.