Quantity
Price
$32.00
Out of stock
Oxytocin
From $32.00to $405.00
For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.
This item is available only through approved account access.
Product Specifications
- Lot Number
- —
- Purity
- —
- Storage Temp
- 36°F to 46°F
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.
Documentation
Certificates of Analysis are supplied with each product lot where applicable.
Certificate of Analysis (COA)
Documentation will be available once product lots are received and tested.
All products currently listed on this site are for research purposes only.
COA Library
Published Certificates of Analysis for Oxytocin. New deliveries are added as their batches are released.
No released COA for this strength yet
Reports appear here once a batch for this strength has been released.
Research Data
Chemical & Structural Identifiers
- Chemical Name (IUPAC)
- L-cysteinyl-L-tyrosyl-L-isoleucyl-L-glutaminyl-L-asparaginyl-L-cysteinyl-L-prolyl-L-leucylglycinamide cyclic (1→6)-disulfide
- CAS Registry Number
- 50-56-6
- PubChem CID
- 439302
- Molecular Formula
- C43H66N12O12S2
- Molecular Weight
- 1006.44 Da (1007.19 g/mol average)
- Sequence
- H-Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2 (Single-letter: CYIQNCPLG-NH2)
- SMILES
- CC[C@H](C)[C@H]1C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CSSC[C@@H](C(=O)N[C@H](C(=O)N1)CC2=CC=C(C=C2)O)N)C(=O)N3CCC[C@H]3C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N)CC(=O)N)CCC(=O)N
- InChIKey
- BVRKZXQLXZBITM-UAKXQUQDSA-N
- Salt / Counter-Ion
- Supplied as a high-purity lyophilized acetate (CH3COO-) or trifluoroacetate (CF3COO-) salt.
- Synonyms
- Oxytocin, Pitocin, Syntocinon, α-Hypophamine, OXT, Oxytocic Hormone, Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2
- Cyclization Architecture: Intramolecular cyclic disulfide bond between cysteine residues at position 1 and position 6 (Cys1–Cys6), forming a 20-membered cyclic hexapeptide ring with a flexible C-terminal tripeptide tail (Pro7-Leu8-Gly9-NH2).
Analytical & COA Specifications
- Analytical Purity (RP-HPLC)
- ≥ 98.5% by area normalization under UV detection at λ = 214 nm and 280 nm (tyrosine chromophore detection).
- Identity Confirmation (ESI-MS / MALDI-TOF)
- Theoretical mass [M+H]+ = 1007.4 Da; observed mass-to-charge ratio m/z = 1007.4 ± 0.5 Da (with confirmed divalent adduct ion [M+2H]2+ = 504.2 Da).
- Residual Moisture (Karl Fischer Titration)
- ≤ 2.0% water content.
- Endotoxin Screening (LAL Assay)
- < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
- Sterility Validation
- USP <71> compliant (no observed microbial proliferation in liquid culture media).
- Physical Characterization
- Dense, white to off-white lyophilized solid cake/powder.
Handling & Stability
Storage. Store dry solid desiccated at -20°C to -80°C, protected from atmospheric moisture and direct light.
Solubility. Freely soluble in sterile laboratory-grade deionized water (≥ 20 mg/mL) and sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 15 mg/mL) for in vitro assay application.
- Disulfide Integrity & Buffer Compatibility: Contains an essential intramolecular disulfide bridge (Cys1–Cys6); *do not* introduce reducing agents (e.g., DTT, TCEP, β-mercaptoethanol) into stock solutions, as reduction of the disulfide bond opens the cyclic ring and abolishes receptor binding affinity. Prepare concentrated working stocks freshly or store single-use aliquots at -20°C to eliminate degradation from repeated freeze-thaw cycles.
Mechanism & Literature
Endogenous cyclic nonapeptide neurohypophysial hormone that acts as a potent, high-affinity full agonist at the G-protein coupled Oxytocin Receptor (OXTR), a Class A (rhodopsin-like) GPCR (Kd ≈ 1.0--2.0 nM).
Cellular Pathways Investigated: Evaluated across in vitro cell models (including primary uterine myometrial cells, mammary myoepithelial cultures, and neuronal cell lines) to quantify:
References (3)
- Du Vigneaud, V., et al. (1953). The synthesis of an octapeptide amide with the hormonal activity of oxytocin. Journal of the American Chemical Society, 75(19), 4879–4880.
- Gimpl, G., & Fahrenholz, F. (2001). The oxytocin receptor system: structure, function, and regulation. Physiological Reviews, 81(2), 629–683.
- Kimura, T., et al. (1992). Structure and expression of a human oxytocin receptor. Nature, 356(6369), 526–529.
Technical Laboratory FAQs
What structural motif is required for Oxytocin biological activity in vitro?
The intramolecular cyclic disulfide bridge between Cys1 and Cys6, combined with the C-terminal glycinamide (Gly9-NH2), forms the conformation necessary to fit into the ligand-binding pocket of the Oxytocin Receptor (OXTR).
Why must reducing agents be strictly omitted during Oxytocin stock preparation?
Thiol reducing agents (such as DTT, TCEP, or 2-mercaptoethanol) cleave the Cys1–Cys6 disulfide bond, converting the cyclic nonapeptide into a linear dithiol chain, which eliminates receptor affinity and causes analytical retention shifts in chromatography.
What solvent conditions are recommended for preparing in vitro Oxytocin working stocks?
Sterile deionized water or sterile PBS (pH 7.4) provides rapid dissolution at concentrations exceeding 15 mg/mL, creating a stable, neutral delivery carrier for cell-based receptor binding and intracellular calcium mobilization assays.
For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.