CJC-1295 DAC

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$28.34

10% off$31.49

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CJC-1295 DAC

Name: CJC-1295 DAC
Brand: KAM Research
Availability: InStock

From $28.34to $212.56

For laboratory research use only. Not for human or animal use, consumption, diagnosis, treatment, or disease prevention.

This item is available only through approved account access.

Product Specifications

Lot Number: —
Purity: —
Storage Temp: 36°F to 46°F

For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.

Documentation

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Certificate of Analysis (COA)

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Published Certificates of Analysis for CJC-1295 DAC. New deliveries are added as their batches are released.

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Research Data

Chemical & Structural Identifiers

Chemical Name (IUPAC): N^{ε 30}-[3-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)propanoyl]-[D-Ala², Gln⁸, Ala¹⁵, Leu²⁷]-GRF(1-29)-Lys³⁰-amide
CAS Registry Number: 446262-90-4 (also cataloged under parent backbone ID 863288-34-0)
PubChem CID: 56841945
Molecular Formula: C₁₆₅H₂₆₉N₄₇O₄₆
Molecular Weight: 3644.02 Da (3647.25 g/mol average)
Sequence: Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-Lys(MPA)-NH₂
SMILES: CC[C@H](C)[C@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@@H](NC(=O)[C@H](Cc2ccccc2)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H](C)NC(=O)[C@H](Cc3ccc(O)cc3)N)[C@@H](C)O)C(=O)NC(CC(C)C)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCCNC(=O)CCN4C(=O)C=CC4=O)C(=O)N
InChIKey: UNQVYJUSWLZTQD-NEXMOMPOSA-N
Salt / Counter-Ion: Supplied as a lyophilized acetate (CH₃COO^-) or trifluoroacetate (CF₃COO^-) salt.
Synonyms: CJC-1295 with DAC, DAC:GRF, CJC-1295 DAC, Maleimidopropionyl-Mod GRF(1-29)

Analytical & COA Specifications

Analytical Purity (RP-HPLC): ≥ 98.5% area normalization under UV detection at λ = 214 nm and 220 nm.
Identity Confirmation (ESI-MS / MALDI-TOF): Theoretical mass [M+H]⁺ = 3648.2 Da; observed mass-to-charge ratio m/z = 3648.2 ± 0.8 Da with confirmed multiple charge distribution states ([M+3H]³⁺, [M+4H]⁴⁺).
Residual Moisture (Karl Fischer Titration): ≤ 3.0% water content.
Endotoxin Screening (LAL Assay): < 1.0 EU/mg (Limulus Amebocyte Lysate per USP <85>).
Sterility Validation: USP <71> compliant (no observed microbial proliferation in fluid thioglycollate or soybean-casein digest broths).
Physical Characterization: High-density, white to off-white lyophilized solid cake/powder.

Handling & Stability

Storage. Store dry solid desiccated at -20^°C to -80^°C, protected from atmospheric moisture and direct light.

Solubility. Soluble in sterile laboratory-grade deionized water (≥ 10 mg/mL), sterile Phosphate-Buffered Saline (PBS, pH 7.4, ≥ 5 mg/mL), or dilute 0.1% acetic acid for stock stabilization in in vitro assay applications.

Maleimide Preservative Handling: The C-terminal maleimide group undergoes rapid nucleophilic addition with free thiols; *do not* reconstitute in buffers containing exogenous reducing agents (e.g., DTT, β-mercaptoethanol, or L-cysteine) unless intentionally conducting bioconjugation reaction assays. Aliquot into single-use fractions to eliminate freeze-thaw degradation cycles.

Mechanism & Literature

Potent Growth Hormone-Releasing Hormone Receptor (GHRHR) agonist engineered with a 3-maleimidopropionic acid (Drug Affinity Complex / DAC) linker at Lys³⁰. In in vitro or biochemical assay media, the maleimide moiety undergoes a spontaneous, selective covalent reaction (Michael addition) with the unpaired cysteine thiol (Cys³⁴) of serum albumin (BSA/HSA).

Cellular Pathways Investigated: Evaluated in primary somatotroph cultures and GHRHR-expressing cell lines to quantify intracellular cyclic AMP (cAMP) accumulation, adenylyl cyclase activation, and stimulation of growth hormone gene transcription (GH1) while bound to albumin adducts. Assays demonstrate selective activation of the pituitary growth hormone axis without inducing non-specific transcription of cortisol or prolactin.

References (2)

Teichman, S. L., et al. (2006). Prolonged stimulation of growth hormone (GH) and insulin-like growth factor I secretion by CJC-1295, a long-acting analog of GH-releasing hormone, in healthy adults. The Journal of Clinical Endocrinology & Metabolism, 91(3), 799–805.
Jette, L., et al. (2005). hGRF1-29-albumin bioconjugates activate the GHRH receptor on anterior pituitary cells in vitro and resist rapid enzymatic degradation. Endocrinology, 146(7), 3052–3058.

Technical Laboratory FAQs

How does CJC-1295 with DAC differ chemically from CJC-1295 No DAC (Mod GRF 1-29)?

While both share the identical tetrasubstituted 29-amino acid GHRH backbone (D-Ala², Gln⁸, Ala¹⁵, Leu²⁷), CJC-1295 DAC contains an additional C-terminal lysine residue functionalized with a 3-maleimidopropionyl group (DAC) for covalent bioconjugation to albumin.

What is the function of the Drug Affinity Complex (DAC) in laboratory assays?

The maleimide functionality on DAC specifically forms a covalent bond with the free sulfhydryl group of Cys³⁴ on albumin, protecting the peptide from enzymatic cleavage by dipeptidyl peptidase-4 (DPP-4) and renal filtration in pharmacokinetic models.

What reconstitution vehicle is recommended for in vitro assay modeling of CJC-1295 DAC?

Sterile deionized water or sterile PBS (pH 7.4) is recommended. Assay buffers must remain free of extraneous thiol-containing reducing reagents to prevent premature quenching of the maleimide reactive group before intended albumin-binding experiments.

For Research Use Only. Not for human or animal administration. Not intended to diagnose, treat, cure, or prevent any disease. Sold for laboratory research purposes only.